Mouse Genome Sequencing

The National Human Genome Research Institute (NHGRI) and the Wellcome Trust currently support a mouse genome sequencing project that aims to produce a robust physical map, and a high quality, finished genomic sequence of the C57BL/6J strain of Mus musculus.

This project is led by the Mouse Genome Sequencing Consortium (MGSC), a collaboration consisting of four large sequencing centers: the Broad Institute/MIT Center for Genomic Research; the Washington University Genome Sequencing Center; the Wellcome Trust Sanger Institute; The Baylor college of Medicine Human Genome Sequencing Center; and an international database, Ensembl, a joint project between the European Bioinformatics Institute and the Sanger Centre.

Project Links:

• The Baylor College of Medicine Human Genome Sequencing Center [hgsc.bcm.tmc.edu]
• Washington University Genome Sequencing Center [genome.wustl.edu]
• Wellcome Trust Sanger Institute [sanger.ac.uk]
• Ensembl [ensembl.org]
• Broad Institute [broadinstitute.org]

Supporting Institutions

• Wellcome Trust [wellcome.ac.uk]

News regarding sequencing, assembly and annotation of the mouse genome can be found in the Mouse Genome Monthly

NHGRI has also convened a group of leading mouse researchers - the Mouse Sequencing Liaison Group - to serve as a line of communication between the sequencing centers and the funding agencies, and the mouse research community.

Membership of this Mouse Sequencing Liaison Group

• Wayne Frankel, Chair - The Jackson Laboratory
• Rudi Balling - Gesellschaft für Biotechnologische Forschung - GBF
• Steve Brown - MRC Mouse Genome Centre
• Maja Bucan - University of Pennsylvania
• Sally Camper - University of Michigan
• David Kingsley - Stanford University
• Janet Rossant - Mt. Sinai Hospital, Toronto
• Eddy Rubin - Lawrence Berkeley Laboratory
• Joseph Takahashi - Northwestern University

The Trans-NIH Mouse Initiative [nih.gov] is a source of information for the development of a wide range of genomic and genetic resources related to the laboratory mouse.

For additional information about the Trans-NIH Mouse Initiative, please contact:
Dr. Bettie Graham
E-mail: Bettie_Graham@nih.gov

Additional Resources

Sequence and Assembly

• Individual Sequence Reads:
  • NCBI Trace Archive [ncbi.nlm.nih.gov]
• Sequence Assemblies:
  • NCBI Mouse Assembly [ncbi.nlm.nih.gov]
    • NCBI MapView [ncbi.nlm.nih.gov/mapview]
  • Genome Gateway at the University of California at Santa Cruz [genome.ucsc.edu]

Model Organism Database

• Mouse Genome Informatics/Database at the Jackson Lab [informatics.jax.org]

Mapping

• Canada's Michael Smith Genome Sciences Centre [bcgsc.ca]
• Washington University Genome Sequencing Center [genome.wustl.edu]

Bacterial Artificial Chromosome (BAC) End Sequencing

• DFCI Mouse Gene Index [compbio.dcfi.harvard.edu]

ESTs

• dbEST - database of ESTs at NCBI [ncbi.nlm.nih.gov]
• Washington University Genome Sequencing Center [genome.wustl.edu]

cDNAs

• Mammalian Gene Collection [mgc.nci.nih.gov]
• RIKEN Institute [osc.riken.jp]

SNPs

• dbSNP [ncbi.nlm.nih.gov] - Database of Single Nucleotide Polymorphisms (SNPs) at NCBI

BAC Libraries

• Children's Hospital Oakland Research Institute (CHORI) [chori.org] Mouse BAC libraries
• Mouse (Multiple Strains) BAC Library Proposal 
• Male C57BL/6J Mouse BAC Library Proposal 
• Deer Mouse BAC Library Proposal 

Other Related Sites

• Cold Spring Harbor Laboratory Mouse Genome Sequencing [mccombielab.cshl.edu]
• University of Oklahoma Mouse Genome Sequencing [genome.ou.edu]