Fungal Genome Sequencing

The National Human Genome Research Institute (NHGRI) has supported and currently supports a series of projects, led by the Broad Institute, aimed at sequencing the genomes of a number of fungi. These fungi were proposed in three individual proposals submitted to NHGRI over the course of three years, consisting of the following sets of organisms.

Whitepaper proposals can be viewed by clicking on the group headings.

• Fungal Genome Initiative I:  
  (Submitted Feb. 2002)
  
  Aspergillus nidulans
  Coccidioides immitis
  Coprinus cinereus
  Cryptococcus neoformans, Serotype A
  Pneumocystis carinii (human and mouse pathogens)
  Rhizopus oryzae
  Ustilago maydis
   
• Fungal Genome Initiative II:  
  (Submitted June 2003)
  
  Candida albicans
  Candida tropicalis
  Lodderomyces elongisporus
  Saccharomyces cerevisiae RM11-1A
   
• Fungal Genome Initiative III:  
  (Submitted Oct. 2003)
  
  Candida guilliermondii
  Candida lusitaniae
  Chaetomium globosum
  Cryptococcus neoformans Serotype B
  Histoplasma capsulatum
  Podospora anserina
  Unicinocarpus reesei
   
• Fungal Genome Initiative IV:  
  (Submitted July 2004)
  
  Batrachochytrium dendrobatidis
  Schizosaccharomyces japonicus
  Schizosaccharomyces octosporus
   
• Geomyces destructans  
  (Submitted June 2009)

For each genome, 10-fold whole genome shotgun sequence coverage from paired-end reads will be obtained from plasmids (90 percent) and fosmids (10 percent). These sequence reads will be used to create a genome assembly.

Project Link:

• The Fungal Genome Initiative [broadinstitute.org]

Additional Resources

Sequence and Assembly

• Individual Sequence Reads
  • NCBI Trace Archive [ncbi.nlm.nih.gov]
     
• Sequence Assembly
  • Aspergillus nidulans [broadinstitute.org]
  • Coprinus cinereus [broadinstitute.org]
  • Cryptococcus neoformans serotype A [broadinstitute.org]
  • Neurospora crassa [broadinstitute.org]
  • Neurospora crassa [ncbi.nlm.nih.gov]
  • Saccharomyces cerevisiae [ncbi.nlm.nih.gov]
  • Ustilago maydis [broadinstitute.org]

ESTs

• dbEST [ncbi.nlm.nih.gov] - Database of ESTs at NCBI